which reason best explains why dead specimens must be used with transmission electron microscopes?\nelectrons…

which reason best explains why dead specimens must be used with transmission electron microscopes?\nelectrons pass over the specimen.\nthe lights that are used are harmful to the specimens.\nspecimens are placed in a vacuum.\nthe image that is produced is two dimensional.
Answer
Brief Explanations:
In transmission electron microscopy, specimens are placed in a vacuum. Living specimens cannot survive in a vacuum environment, so dead specimens must be used. The other options do not directly relate to why specimens have to be dead.
Answer:
Specimens are placed in a vacuum.