the segment of dna shown in the figure above has restriction sites i and ii, which create restriction…
the segment of dna shown in the figure above has restriction sites i and ii, which create restriction fragments a, b, and c. which of the gels produced by electrophoresis shown below best represents the separation and identity of these fragments?
Answer
Brief Explanations:
In gel - electrophoresis of DNA, smaller fragments move faster towards the positive electrode. Fragment C is the largest as it is not cut by either restriction site, fragment A is the smallest as it is the most cut - off piece. So, fragments should be separated with C being closest to the negative electrode and A being closest to the positive electrode.
Answer:
The gel with fragments arranged as C, B, A from the negative to the positive electrode.